Dr. John C. Wallace

Physcomitrella patens
EDUCATION: A.B. cum laude in Chemistry and Biology, Harvard University, Cambridge MA, 6/75.
Ph.D. in Biochemistry, University of Pittsburgh, 8/83.

EMPLOYMENT: Research Assistant in respiratory toxicology laboratory, Dept. of Industrial Environmental Health Sciences, Graduate School of Public Health, University of Pittsburgh, 9/75 - 8/77.
Teaching Assistant and Graduate Research Assistant, Dept. of Biological Sciences, University of Pittsburgh, 9/77 - 5/83.
Postdoctoral Fellow, Institut de Chimie Biologique, Faculte de Medecine, Strasbourg, France 9/83 - 12/85.
Postdoctoral Fellow, Dept. of Botany and Plant Path, Purdue Univ, 2/86 - 5/89.
Assistant Professor, Dept. of Biology, Bucknell University, PA 9/89 - 8/95.
Associate Research Professor, Dept. of Plant Biology, University of New Hampshire, 8/96 - present.
AWARDS: Postdoctoral Fellowship, American Cancer Society, 1/84 - 12/85.
Postdoctoral Fellowship, Plant Molecular Biology, National Science Foundation, 6/86 - 5/89.

RESEARCH GRANTS AND PROPOSALS: Proposal to National Science Foundation (REU program) entitled "Summer Research in Cell and Molecular Biology for Undergraduates" funded for $ 40,300.
Proposal to US Dept. of Agriculture (Seed grant program) entitled "Antisense tagging to clone developmentally important genes in the moss P. Patens," funded for $ 50,000.

PROFESSIONAL SOCIETIES: International Society for Plant Molecular Biology.
American Society of Plant Physiologists.

Upper level: Developmental Biology
Biochemical Methods
Molecular Biology
Plant Growth and Development
First year: Cell and Molecular Biology
Genetics and Development
Biotechnology (non-majors)
Genetics and Evolution

Graduate work was on the isolation and characterization of RNA populations from Hela cells. Most notably I discovered the branch structure in nuclear RNA molecules which was subsequently shown to be part of the "lariat" intermediate in pre-mRNA splicing. I also characterized an oligo(U)-containing subpopulation of HeLa cell mRNA according to its sequence complexity, cap structure, and kinetics of synthesis and turnover. My major advisor was Prof. Mary Edmonds. My thesis (University of Pittsburgh, 1983) was entitled "Analyses of Cytoplasmic Polyadenylated Oligo(uridylic acid)-containing RNA from Hela Cells, and the Discovery of Branches in Nuclear Polyadenylated RNA." Postdoctoral work in Strasbourg involved the construction of recombinant DNA clones, their introduction into cultured mammalian cells, and analysis of their expression. The emphasis was on examination of DNA sequences responsible for estrogen-induced stimulation of transcription. Mostly while in France I became familiar with the (then new) methodology of recombinant DNA. I was in Pierre Chambon's laboratory. My work with Brian Larkins at Purdue centered around zeins, the storage proteins of maize. Emphasis was on the use of genetic engineering techniques to improve the nutritional quality of maize, and I also investigated mechanisms of control of expression of these proteins. At Bucknell I continued my work with the zeins, but have now switched into research on the molecular aspects of development of the moss Physcomitrella patens, with particular emphasis on transformation and rescue techniques to isolate genes of developmental interest. The spring semester of 1992-93 was spent at the University of Leeds in England in the laboratory of Dr. David Cove, where I became familiar with the Physcomitrella system, and in particular worked on transformation of the moss via particle bombardment and characterization of the resultant transformants. Technical Expertise: I have published papers in which I have employed the following techniques: nucleic acids purification and various chemical and biochemical analyses; construction and screening of genomic and cDNA libraries; various aspects of subcloning and creation of recombinant DNA constructs; site-directed mutagenesis; southern, northern, and dot-blot analyses; mammalian cell transfection; SDS-PAGE; isoelectric focusing; development of immunological (ELISA) assays; S1 mapping; manual DNA sequencing; and plant transformation via particle bombardment. I also have at least some experience with the following: PCR; differential display PCR; Agrobacterium-mediated transformation; western blotting; Xenopus oocyte injection; antisense technology; electroporation (prokaryotic and eukaryotic cells); plant protoplast production, transformation and regeneration.
John Wallace and Yves Alarie (1978). The Interactions of Sensory Irritants with a Glutathione S-Transferase Activity from Bovine Corneal Epithelium. Tox. Applied Pharm. 45, 358.

John C. Wallace, William M. Wood, and Mary Edmonds (1981). 5' Terminal Cap Structures of Oligo(uridylic acid)-Containing Messenger RNA from HeLa Cells. Biochemistry 20, 5364-5368.

John C. Wallace and Mary Edmonds (1983). Polyadenylated Nuclear RNA Contains Branches. Proc. Nat. Acad. Sci. (USA) 80, 950-954.

William M. Wood, John C. Wallace, and Mary Edmonds (1985). Sequence Content of Oligo(uridylic acid)-containing Messenger RNA from HeLa Cells. Biochemistry 24, 3686-3693.

Margo Roberts, John Wallace, Jean-Marc Jeltsch, and Meera Berry (1988). The 5' Flanking Region of the Human pS2 Gene Mediates its Transcriptional Activation by Estrogen in MCF-7 Cells. Biochem. Biophys. Res. Comm. 151, 306-313.

John C. Wallace, Gad Galili, Evelynn E. Kawata, Richard E. Cuellar, Mark A. Shotwell, and Brian A. Larkins (1988). Aggregation of Lysine-containing Zeins into Protein Bodies in Xenopus Oocytes. Science 241, 662-664.

John C. Wallace, G. Galili, E.E. Kawata, C.R. Lending, A.L. Kriz, C.E. Bracker, and B.A. Larkins (1988). Location and Interaction of the Different Types of Zeins in Protein Bodies. Biochem. Physiol. Pflanzen 183, 107-115.

Brian A. Larkins, Craig R. Lending, John C. Wallace, Gad Galili, Evelynn E. Kawata, Kalyanram B. Geetha, Alan L. Kriz, David N. Martin, and Charles E. Bracker (1988). Zein Gene Expression during Maize Endosperm Development. In "The Molecular Basis of Plant Development," (Goldberg, R.B., ed.) Alan R. Liss, 109-120.

Brian A. Larkins, John C. Wallace, Gad Galili, Craig R. Lending, and Evelynn E. Kawata (1989). Structural Analysis and Modification of Maize Storage Proteins. Developments in Industrial Microbiology, vol. 30 (J. Industrial Microbiol., Suppl. No. 4)(J. Cooney and O. Sebek, eds.). Society for Industrial Microbiology, 203-209 .

Brian A. Larkins, John C. Wallace, Gad Galili, Craig R. Lending, and Evelynn E. Kawata (1989). Characterization and modification of maize storage proteins. In "Biotechnology and Food Quality" (S.D. Kung, D.D. Bills, and R. Quatrano eds.). Butterworths, 67-81.

J. David Reilly, John C. Wallace, and Mary Edmonds (1987). The quantitation and distribution of splicing intermediates in HeLa cells and adenovirus RNAs. Nucleic Acids Res. 15, 7103-7124.

J. David Reilly, John C. Wallace, Randa F. Melhem, David W. Kopp, and Mary Edmonds (1989). Isolation and Characterization of Branched Oligonucleotides from RNA. Methods in Enzymology 180, 177-191.

John C. Wallace, Takeshi Ohtani, Craig R. Lending, Mauricio Lopes, John D. Williamson, Katy L. Shaw, Stanton B. Gelvin, and Brian A. Larkins (1990). Factors affecting physical and structural properties of maize protein bodies. In "Plant Gene Transfer," UCLA Symposia on Molecular and Cellular Biology, New Series, Volume 129 (Lamb, C. and Beachy, R, eds.). Alan R. Liss, 205-216.

John C. Wallace, Mauricio A. Lopes, Edilson Paiva, and Brian A. Larkins (1990). New methods for extraction and quantitation of zeins reveal a high content of gamma-zein in modified opaque-2 maize. Plant Physiology 92, 191-196.

Brian A. Larkins, Craig R. Lending, John C. Wallace, Gad Galili, and Mauricio A. Lopes (1990). Application of biotechnology for improving cereal protein quality. Proceedings of the International Conference on Nutritional Improvement of Sorghum. Purdue University Press, 155-163.

Takeshi Ohtani, Gad Galili, John C. Wallace, Gary A. Thompson and Brian A. Larkins (1991). Normal and lysine-containing zeins are unstable in transgenic tobacco seeds. Plant Molecular Biology 16, 117-128.

Edilson Paiva, Alan L. Kriz, Maria J.V.V.D. Peixoto, John C. Wallace, and Brian A. Larkins (1991). Quantitation and Distribution of gamma-Zein in the Endosperm of Maize Kernels. Cereal Chemistry 68, 276-279.

K.B. Geetha, Craig R. Lending, Mauricio A. Lopes, John C. Wallace, and Brian A. Larkins (1991). Opaque-2 Modifiers Increase gamma-Zein Synthesis and Alter Its Spatial Distribution in Maize Endosperm. The Plant Cell 3, 1207-1219.

Craig R. Lending, John C. Wallace, and Brian A. Larkins (1992). Synthesis of Zeins and Their Potential for Amino Acid Modification. In "Plant Protein Engineering," (S. Gutteridge and P. Shewry, eds.), 209-218.

Brian A. Larkins, Craig R. Lending, and John C. Wallace (1993). Modification of maize-seed-protein quality. Am. J. Clin. Nutr. 58(suppl), 264S-269S.

John C. Wallace (1994). Developmental Genetics in Lower Plants. In "Handbook of Plant and Crop Physiology," (M. Pessarakli, ed.), 771-784.

John C. Wallace, Celia D. Knight, and Wagdy Sawahel. Maintenance of non-selected transforming DNA in unstable transformants of the moss, Physcomitrella patens. Submitted to Plant Science.

Celia D. Knight, Amita Sehgal, Kamaljit Atwal, John C. Wallace, David J. Cove, David Coates, Ralph S. Quatrano, Sultan Bahadur, Peter G. Stockley, and Andrew C. Cuming (1995). Molecular responses to abscisic acid and stress are conserved between moss and cereals. The Plant Cell 7, 499-506.

Speaking and reading knowledge of French, able to get around (barely) in German.

I am tall, healthy, a U.S. citizen, married, born 3/29/53. My hobbies include music (choral singing, 'cello, recorder), sports (tennis, squash, bicycling), gardening, and the outdoors (hiking, backpacking, cross-country skiing). I also enjoy going to concerts and the theater, and sharing the appreciation of good food, wine, and beer with friends. I occasionally brew beer and make wine. I am an active environmentalist: I belong to several environmental organizations, serve on the board of directors of our local Audubon Society, and serve on a "Greening of Bucknell" task force. My wife, Dr. Lessa Brill, is a gynecologist, and we have adopted kids, Penina Raquel Wallace, born May 1991, and Simon Roy Wallace, July 1995.
You can reach me by e-mail at: jw2@hopper.unh.edu